Alpha-amylase is used in the starch industry to continuously liquefy starch, wet-milled corn, and wheat starch for the production of low dextrose starch syrups. In this step, mash from corn or other starches is first cooked down to a gravy-like substance. Then, the liquefaction process begins with partial hydrolysis to lower its viscosity.

Saccharification is the process of breaking down complex carbohydrates like corn or sugar cane into monosaccharide components in the ethanol fuel production process and Whole saccharification process was done by Glucoamylase and pullulnase enzyme.